rabbit polyclonal antibody against pept1 Search Results


slc15a1  (Bioss)
93
Bioss slc15a1
Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and <t>SLC15A1</t> ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.
Slc15a1, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pmc08532950-79-21-25?v=Bioss
Average 93 stars, based on 1 article reviews
slc15a1 - by Bioz Stars, 2026-07
93/100 stars
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gene synthesis  (Integrated DNA Technologies)
94
Integrated DNA Technologies gene synthesis
Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and <t>SLC15A1</t> ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.
Gene Synthesis, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/custom%40gene-synthesis%4028943923?v=Integrated+DNA+Technologies
Average 94 stars, based on 1 article reviews
gene synthesis - by Bioz Stars, 2026-07
94/100 stars
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pept1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology pept1
( A ) Study by immunocytofluorescence to assess the cellular localization of P-glycoprotein ( red ), MRP1 ( red ), and <t>PepT1</t> ( red ) in NF and HF. Nuclei were visualized with DAPI ( blue ). White arrowheads point to the positive immunostainings. Scale bar: 25 μm. ( B ) Immunoblotting of P-glycoprotein, MRP1 and PepT1 in the lysates of NF and HF. β-actin served as an equal protein loading control. Histogram compared the difference on the relative protein expression between NF and HF. Each data point was normalized against corresponding β-actin with the average value after normalization in NF arbitrarily set as 1. Error bars represent means ± SD ( n = 6), * p < 0.05, ** p < 0.01.
Pept1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pmc05356828-24-0-6?v=Santa+Cruz+Biotechnology
Average 94 stars, based on 1 article reviews
pept1 - by Bioz Stars, 2026-07
94/100 stars
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rabbit polyclonal anti pept 1 antibody  (Danaher Inc)
99
Danaher Inc rabbit polyclonal anti pept 1 antibody
( A ) Study by immunocytofluorescence to assess the cellular localization of P-glycoprotein ( red ), MRP1 ( red ), and <t>PepT1</t> ( red ) in NF and HF. Nuclei were visualized with DAPI ( blue ). White arrowheads point to the positive immunostainings. Scale bar: 25 μm. ( B ) Immunoblotting of P-glycoprotein, MRP1 and PepT1 in the lysates of NF and HF. β-actin served as an equal protein loading control. Histogram compared the difference on the relative protein expression between NF and HF. Each data point was normalized against corresponding β-actin with the average value after normalization in NF arbitrarily set as 1. Error bars represent means ± SD ( n = 6), * p < 0.05, ** p < 0.01.
Rabbit Polyclonal Anti Pept 1 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pmc07435111-132-1-5?v=Danaher+Inc
Average 99 stars, based on 1 article reviews
rabbit polyclonal anti pept 1 antibody - by Bioz Stars, 2026-07
99/100 stars
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peptide transporter 1 pept1  (Bioss)
92
Bioss peptide transporter 1 pept1
( A ) Study by immunocytofluorescence to assess the cellular localization of P-glycoprotein ( red ), MRP1 ( red ), and <t>PepT1</t> ( red ) in NF and HF. Nuclei were visualized with DAPI ( blue ). White arrowheads point to the positive immunostainings. Scale bar: 25 μm. ( B ) Immunoblotting of P-glycoprotein, MRP1 and PepT1 in the lysates of NF and HF. β-actin served as an equal protein loading control. Histogram compared the difference on the relative protein expression between NF and HF. Each data point was normalized against corresponding β-actin with the average value after normalization in NF arbitrarily set as 1. Error bars represent means ± SD ( n = 6), * p < 0.05, ** p < 0.01.
Peptide Transporter 1 Pept1, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pm31385432-23-8-15?v=Bioss
Average 92 stars, based on 1 article reviews
peptide transporter 1 pept1 - by Bioz Stars, 2026-07
92/100 stars
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rabbit anti human pept1  (Boster Bio)
93
Boster Bio rabbit anti human pept1
Primer sequences for real-time PCR amplification.
Rabbit Anti Human Pept1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pmc09355254-132-95-99?v=Boster+Bio
Average 93 stars, based on 1 article reviews
rabbit anti human pept1 - by Bioz Stars, 2026-07
93/100 stars
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polyclonal canine pept1  (Biorbyt)
85
Biorbyt polyclonal canine pept1
Primer sequences for real-time PCR amplification.
Polyclonal Canine Pept1, supplied by Biorbyt, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pmc03618282-169-0-11?v=Biorbyt
Average 85 stars, based on 1 article reviews
polyclonal canine pept1 - by Bioz Stars, 2026-07
85/100 stars
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sds polyacrylamide gel  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology sds polyacrylamide gel
Primer sequences for real-time PCR amplification.
Sds Polyacrylamide Gel, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pmc05064774-64-6-69?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
sds polyacrylamide gel - by Bioz Stars, 2026-07
96/100 stars
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sglt1/slc5a1 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation sglt1/slc5a1 antibody
Primer sequences for real-time PCR amplification.
Sglt1/Slc5a1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/custom%40nbp2-20338%4010%2E21203%2Frs%2E3%2Ers-164747?v=Bio-Techne+corporation
Average 94 stars, based on 1 article reviews
sglt1/slc5a1 antibody - by Bioz Stars, 2026-07
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bs-0038r  (Bioss)
94
Bioss bs-0038r
Primer sequences for real-time PCR amplification.
Bs 0038r, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/custom%40bs-0038r%4010%2E21203%2Frs%2E3%2Ers-164747?v=Bioss
Average 94 stars, based on 1 article reviews
bs-0038r - by Bioz Stars, 2026-07
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anti-gipr (1:200)  (Becton Dickinson)
90
Becton Dickinson anti-gipr (1:200)
Primer sequences for real-time PCR amplification.
Anti Gipr (1:200), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pept1/pmc03840233-179-18-23?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti-gipr (1:200) - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and SLC15A1 ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.

Journal: Antioxidants

Article Title: The Cytotoxicity of Doxorubicin Can Be Accelerated by a Combination of Hyperthermia and 5-Aminolevulinic Acid

doi: 10.3390/antiox10101531

Figure Lengend Snippet: Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and SLC15A1 ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.

Article Snippet: Antirabbit SLC22A16 (cat. no. LS-C176922) (LSBio, Seattle, WA, USA), ABCG2 (cat. no. 42078) (Cell Signaling Technology Japan K.K., Tokyo, Japan), or SLC15A1 (cat. no. bs-10588R) (Bioss, Boston, MA, USA) antibodies (1:1000) were added to the Can Get Signal ® Immunoreaction Enhancer Solution 1 (Toyobo Co. Ltd., Osaka, Japan).

Techniques: Western Blot, Expressing

( A ) Study by immunocytofluorescence to assess the cellular localization of P-glycoprotein ( red ), MRP1 ( red ), and PepT1 ( red ) in NF and HF. Nuclei were visualized with DAPI ( blue ). White arrowheads point to the positive immunostainings. Scale bar: 25 μm. ( B ) Immunoblotting of P-glycoprotein, MRP1 and PepT1 in the lysates of NF and HF. β-actin served as an equal protein loading control. Histogram compared the difference on the relative protein expression between NF and HF. Each data point was normalized against corresponding β-actin with the average value after normalization in NF arbitrarily set as 1. Error bars represent means ± SD ( n = 6), * p < 0.05, ** p < 0.01.

Journal: Oncotarget

Article Title: Disruption of the association between drug transporter and actin cytoskeleton abolishes drug resistance in hypertrophic scar

doi: 10.18632/oncotarget.13734

Figure Lengend Snippet: ( A ) Study by immunocytofluorescence to assess the cellular localization of P-glycoprotein ( red ), MRP1 ( red ), and PepT1 ( red ) in NF and HF. Nuclei were visualized with DAPI ( blue ). White arrowheads point to the positive immunostainings. Scale bar: 25 μm. ( B ) Immunoblotting of P-glycoprotein, MRP1 and PepT1 in the lysates of NF and HF. β-actin served as an equal protein loading control. Histogram compared the difference on the relative protein expression between NF and HF. Each data point was normalized against corresponding β-actin with the average value after normalization in NF arbitrarily set as 1. Error bars represent means ± SD ( n = 6), * p < 0.05, ** p < 0.01.

Article Snippet: PepT1 , sc-20653 , Rabbit , Santa cruz biotechnology , 1:200 , 1:50 , , , .

Techniques: Western Blot, Control, Expressing

( A ) Study by immunohistochemistry to assess the distribution of P-glycoprotein ( brownish ), MRP1 ( brownish ), and PepT1 ( brownish ) in normal dermis (ND) and hypertrophic scar dermis (HD). Nuclei were conterstained with hematoxylin ( blue ). Black arrowheads point to the positive immunostainings. Scale bar: 50 μm. ( B ) Histogram comparing the P-glycoprotein-, MRP1-, or PepT1-positively stained cell rate between ND and HD. ( C ) Immunoblotting of P-glycoprotein, MRP1 and PepT1 in the lysates from ND and HD. β-actin served as an equal protein loading control. Histogram compared the difference on the relative protein expression between ND and HD. Each data point was normalized against corresponding β-actin with the average value after normalization in NF arbitrarily set as 1. Error bars represent means ± SD ( n = 6), ** p < 0.01.

Journal: Oncotarget

Article Title: Disruption of the association between drug transporter and actin cytoskeleton abolishes drug resistance in hypertrophic scar

doi: 10.18632/oncotarget.13734

Figure Lengend Snippet: ( A ) Study by immunohistochemistry to assess the distribution of P-glycoprotein ( brownish ), MRP1 ( brownish ), and PepT1 ( brownish ) in normal dermis (ND) and hypertrophic scar dermis (HD). Nuclei were conterstained with hematoxylin ( blue ). Black arrowheads point to the positive immunostainings. Scale bar: 50 μm. ( B ) Histogram comparing the P-glycoprotein-, MRP1-, or PepT1-positively stained cell rate between ND and HD. ( C ) Immunoblotting of P-glycoprotein, MRP1 and PepT1 in the lysates from ND and HD. β-actin served as an equal protein loading control. Histogram compared the difference on the relative protein expression between ND and HD. Each data point was normalized against corresponding β-actin with the average value after normalization in NF arbitrarily set as 1. Error bars represent means ± SD ( n = 6), ** p < 0.01.

Article Snippet: PepT1 , sc-20653 , Rabbit , Santa cruz biotechnology , 1:200 , 1:50 , , , .

Techniques: Immunohistochemistry, Staining, Western Blot, Control, Expressing

Summary of primary antibodies used in this study

Journal: Oncotarget

Article Title: Disruption of the association between drug transporter and actin cytoskeleton abolishes drug resistance in hypertrophic scar

doi: 10.18632/oncotarget.13734

Figure Lengend Snippet: Summary of primary antibodies used in this study

Article Snippet: PepT1 , sc-20653 , Rabbit , Santa cruz biotechnology , 1:200 , 1:50 , , , .

Techniques:

Primer sequences for real-time PCR amplification.

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Primer sequences for real-time PCR amplification.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Real-time Polymerase Chain Reaction, Amplification, Sequencing

Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, ( n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, ( n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing